Figure 8From: Enzyme affinity to cell types in wheat straw (Triticum aestivum L.) before and after hydrothermal pretreatmentEnzyme affinity towards purified lignin. LM (1) and CLSM images (2, 3) of purified lignin incubated with a fluorescently labelled endoglucanase (A) or exoglucanase (B). Each row shows one identical motive taken with the same objective (4x). CLSM images were captured using the same laser intensity of 20% but with different gains, i.e. 500 V (2) and 700 V (3), respectively. Notice that no emission is detected at ‘standard’ 500 V, only after a significant increase in gain to 700 V.Back to article page