Figure 4

Malate synthase activity and glyoxylate bioconversion into butanol and isobutanol. (A) Glyoxylate conversion reaction performed by malate synthase enzyme in the presence of butyryl-CoA. The carbons of the molecules were numbered. (B) Malate synthase activity for the glyoxylate conversion into β-ethylmalate. Left panel: malate synthase activity was assayed using acetyl-CoA or butyryl-CoA as donor group. Right panel: effect of MLS1 deletion on the enzymatic activity. The data presented here are representative of three independent experiments. p ≤ 0,05 = *; p ≤ 0,01 = **; p ≤ 0,001 = ***; p > 0,05 = n.s. (C) Glyoxylate bioconversion. Butanol (full columns) and isobutanol (empty columns) production as well as glyoxylate consumption (triangle) measured at different time point. The data presented here are representative of three independent experiments.