Figure 3

Growth curves for the adapted Saccharomyces cerevisiae strain engineered to express the P. ruminicola D-xylose isomerase and D-xylulokinase genes. A) Strains were cultured under aerobic conditions in YP medium with 50 g/L D-xylose. Cultures were incubated at 30°C, shaking at 1000 rpm using a BioLector®. Cell density was measured every 30 minutes. Data shown are mean values from experiments performed in triplicate. B) Strains were cultured under microaerobic conditions using YP medium with 50 g/L D-xylose. Cultures were incubated at 30°C using a Bioscreen C™. Cell density was measured every 30 minutes. Data shown are the average values from three biological replicates. The standard deviation for most values was less than 5%. Panel B uses the same legend as in panel A.