Growth and fermentation of D-xylose by Saccharomyces cerevisiae expressing a novel D-xylose isomerase originating from the bacterium Prevotella ruminicola TC2-24

Hector, Ronald E; Dien, Bruce S; Cotta, Michael A; Mertens, Jeffrey A

doi:10.1186/1754-6834-6-84

Biotechnology for Biofuels and Bioproducts

Table 4 Microorganisms used in this study

From: Growth and fermentation of D-xylose by Saccharomyces cerevisiae expressing a novel D-xylose isomerase originating from the bacterium Prevotella ruminicola TC2-24

Strain	Genotype (description)	Reference
V601	Bacteroides uniformis	This work
V923	Bacteroides distasonis	This work
V975	Bacteroides ovatus	This work
TC2-24	Prevotella ruminicola (NRRL # B-50773)	This work
TC27	Prevotella ruminicola	This work
D31d	Prevotella ruminicola	This work
20-63	Prevotella ruminicola	This work
20-78	Prevotella ruminicola	This work
E40a	Prevotella ruminicola	This work
E42g	Prevotella ruminicola	This work
H15a	Prevotella ruminicola	This work
H2b	Prevotella ruminicola	This work
118b	Prevotella ruminicola	This work
20-92A	Prevotella ruminicola	This work
CEN.PK2-1C	S. cerevisiae MAT a ura3-52 trp1-289 leu2-3,112 his3 Δ1 MAL2-8^CSUC2	Euroscarf
YRH400	D5A + integrated (KanMX4; P_PGK1-XYL1-T_PGK1; P_ADH1-XYL2-T_ADH1; P_HXT7-XKS1-T_HXT7)	[27]
YRH561	CEN.PK2-1C [pRS414, pRS416] (control strain with empty vectors)	This work
YRH562	CEN.PK2-1C [pRH195, pRH218] (low copy XKS1, high copy Piromyces XI)	This work
YRH563	CEN.PK2-1C [pRH195, pRH351] (low copy XKS1, high copy B. uniformis XI)	This work
YRH564	CEN.PK2-1C [pRH195, pRH352] (low copy XKS1, high copy B. distasonis XI)	This work
YRH565	CEN.PK2-1C [pRH195, pRH353] (low copy XKS1, high copy B. ovatus XI)	This work
YRH587	CEN.PK2-1C [pRH195, pRH367] (low copy XKS1, high copy P. ruminicola XI)	This work
YRH592	CEN.PK2-1C [pRH351, pRH369] (low copy B. uniformis XK, high copy B. uniformis XI)	This work
YRH628	CEN.PK2-1C [pRH384] (no XKS1, high copy P. ruminicola XI^a)	This work
YRH629	CEN.PK2-1C [pRH195, pRH384] (low copy XKS1, high copy P. ruminicola XI^a)	This work
YRH630	CEN.PK2-1C [pRH385, pRH218] (low copy P. ruminicola XK^a, high copy Piromyces XI)	This work
YRH631	CEN.PK2-1C [pRH385, pRH384] (low copy P. ruminicola XK^a, high copy P. ruminicola XI^a)	This work
YRH1114	YRH631 adapted for improved D-xylose fermentation	This work
YRH1136	YRH1114 with XI and XK vectors (pRH384 and pRH385) evicted	This work
YRH1137	YRH114 with XI vector (pRH384) evicted, maintains the P. ruminicola XK^a [pRH385]	This work
YRH1138	YRH114 with XK vector (pRH385) evicted, maintains the P. ruminicola XI^a [pRH384]	This work

^a codon adapted for expression in S. cerevisiae.

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ISSN: 2731-3654

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