Figure 4From: Identification of multiple interacting alleles conferring low glycerol and high ethanol yield in Saccharomyces cerevisiae ethanolic fermentationLinkage analysis of QTLs on chr. II, IV and XIII with different groups of segregants. (a) Glycerol and ethanol yield (on glucose) of the parental strains, 26B (■) and ER7A (▲), and the hybrid diploid strain 26B/ER7A (●). Glycerol and ethanol yield of the first isolated F1 segregants from 26B/ER7A (○), of the additional F1 segregants (□) and of the F5 segregants (◊). For screening purposes, one fermentation was carried out in 5 ml YP 10% glucose. Glycerol and ethanol yield of all segregants, ER7A and the diploid 26B/ER7A were related to the yield of 26B, which was set as 100% (b) Segregants were selected for low glycerol (<120% glycerol yield, stippled line) and high ethanol (>99% ethanol yield, stippled line) yield (on glucose) after each round of screening, resulting in the following segregant groups: 22 F1 segregants used for pooled-segregant whole-genome sequence analysis (○), 22 additional selected F1 segregants (□), and 26 F5 segregants (◊). These segregants were reconfirmed in 100 ml YP 10% glucose. Values for glycerol and ethanol yield are the average of three replicates. (c) SNP variant frequency (top) and respective P-value (bottom) were determined by allele-specific PCR in individual segregants of the sequenced selected pool (●), additional F1 selected pool (○), the total F1 selection of 44 (▲), the selection of F5 segregants (△), and the total selection of all 70 segregants (■) to fine-map the QTLs on chr. II, IV and XIII, which were detected with EXPloRA. The statistical confidence line (for P-value ≤ 0.05) is indicated with a stippled line.Back to article page