Figure 3From: A novel thermostable xylanase GH10 from Malbranchea pulchella expressed in Aspergillus nidulans with potential applications in biotechnologyThe effect of glycosylation on MpXyn10A and structural analyses. (A) Analyses of MpXyn10A glycosylation by SDS-PAGE. L - ladder; 1 - MpXyn10A treated with endoglycosidase H (Endo H) and purified; 2 - untreated MpXyn10A. (B) Differential scanning fluorimetry of glycosylated (solid squares) and deglycosylated (open circles) MpXyn10A. The melting temperature was estimated at 80.34°C for the glycosylated enzyme, with R2 = 0.9956, and 80.13°C for the deglycosylated enzyme, with R2 = 0.9975. (C) Three-dimensional structure of MpXyn10A generated by molecular modeling. Black arrows indicate the predicted glycosylation sites. (D) Far-UV circular dichroism spectrum of MpXyn10A. See Methods section for further experimental details.Back to article page