Figure 1From: Fourier transform infrared spectroscopy as a method to study lipid accumulation in oleaginous yeastsGrowth, microscopy and flow cytometry. (A) Growth curves of S. cerevisiae (square), C. curvatus (triangle), R. toruloides (circle) in rich malt extract, soytone (MS) medium (30 g/l malt extract, 3 g/l soytone). (B) S. cerevisiae C. curvatus and R. toruloides cells were stained with Nile red (9-diethylamino-5H-benzo[α]phenoxazine-5-one) after 72 h (T72) of growth and observed under a microscope. (C) Flow cytometric analysis of S. cerevisiae, C. curvatus and R. toruloides after 0 (T0) and 72 h after inoculum in rich medium. Cells were washed twice and stained for 5 minutes with Nile red before flow cytometric analysis. X mean, values representing the medium fluorescence of S. cerevisiae C. curvatus, and R. toruloides. OD, optical density.Back to article page