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Table 5 Primers for the quantitative PCR analysis

From: Screening of candidate regulators for cellulase and hemicellulase production in Trichoderma reesei and identification of a factor essential for cellulase production

Gene

5′ primer

3′ primer

cbh1

GCGGATCCTCTTTCTCAG

ATGTTGGCGTAGTAATCATCC

cbh2

TCCTGGTTATTGAGCCTGAC

GCAACATTTGGAAGGTTCAG

egl1

GTCTACTACGAACTCGAC

GTAGTAGTCGTTGCTATACTG

bgl1

GCCTCCAAGATCAGCTATCC

ACCTCCTCACCGATGAACTG

xyn1

AAACTACCAAACTGGCGG

TTGATGGGAGCAGAAGATCC

xyn2

CGGCTACTTCTACTCGTACTG

TTGATGACCTTGTTCTTGGTG

xyn3

TACAAGGGCAAGATTCGTG

ACTGGCTTCCAATACCGT

axe1

TAAAGCAGCAATCTTCATGG

GCAGTAAGACTTGATCTTGG

bxl1

GTCACTCTTCCAAGCTCAG

ATCGTTACCTCTTCTCCCA

xyr1

GAGTATCAGCGCAACTTTAGCA

CATCGGTATAGTGCAAGAAGCTC

sar1

TCTCCACCCTACTTCTGAG

CTTGTTGCCCAGGATGAC

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Biotechnology for Biofuels and Bioproducts

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