Figure 2

A plot highlighting the correlation between thermotolerance and ionic liquid (IL)-tolerance of the enzymes shown in Table3. The plot shows the maximum [C2mim][OAc] concentration that permits ≥80% enzyme activity compared to water versus the optimum temperature (T _opt ) of the enzyme. There are two overlapping data points at 95°C, 35% IL. Enzymes with high IL-tolerance are defined as the enzymes that can tolerate 20% (v/v) [C2mim][OAc] or greater (above horizontal line). The enzymes fall into two clusters: the black polygon where 78% (7/9) of the enzymes with a T _opt >70°C have high IL-tolerance, and the grey polygon where 82% (9/11) of the enzymes with a T _opt ≤70°C have low or no IL-tolerance. Only 18% (2/11) of the enzymes with a T _opt ≤70°C have high IL-tolerance.