Figure 4From: Determinants for the improved thermostability of a mesophilic family 11 xylanase predicted by computational methodsTemperature optimum and stability of the recombinant xylanases. (A) The temperature optima were measured under the standard assay conditions, but temperatures ranged from 35 to 60°C for reAuXyn11A as well as from 45 to 80°C for reAEXynM and its recombinant mutants. (B) To estimate the temperature stabilities, reAuXyn11A and reAEXynMC5T were incubated from 35 to 60°C and other recombinant xylanases from 45 to 80°C, respectively, for 1.0 hour. ■, reAuXyn11A; ▲, reAEXynM; □, reAEXynMC5T; △, reAEXynMP9S; ○, reAEXynMH14N.Back to article page