Superior triacylglycerol (TAG) accumulation in starchless mutants of Scenedesmus obliquus: (I) mutant generation and characterization

Table 1 Volumetric productivities of S. obliquus wild type and five selected starchless mutants

		Volumetric biomass productivity (mg L^-1 day^-1)		Volumetric TFA productivity (mg L^-1 day^-1)		Volumetric TAG productivity (mg L^-1 day^-1)
		Average	Maximum	Average	Maximum	Average
Wild type	N+	295 ± 2	334 ± 25	30 ± 2	42 ± 2	4 ± 0
Wild type	N-	140 ± 5	255^b ± 4	104 ± 5	138 ± 5	95 ± 3
Slm 1	N+	271 ± 1	390 ± 24	35 ± 1	44 ± 2	3 ± 0
Slm 1	N-	166^b ± 2	222 ± 20	125^b ± 2	173^b ± 9	112^b ± 3
Slm 2	N+	322^a ± 5	362 ± 24	42^a ± 5	54 ± 7	12^a ± 1
Slm 2	N-	146 ± 0	182 ± 22	101 ± 0	145 ± 2	94 ± 1
Slm 3	N+	307 ± 1	360 ± 9	42 ± 1	57^a ± 1	3 ± 0
Slm 3	N-	147 ± 1	240 ± 69	107 ± 1	170 ± 14	98 ± 2
Slm 4	N+	269 ± 2	408^a ± 87	35 ± 2	46 ± 4	4 ± 0
Slm 4	N-	137 ± 1	208 ± 24	105 ± 1	140 ± 6	95 ± 0
Slm 6	N+	278 ± 2	322 ± 8	35 ± 2	46 ± 4	7 ± 0
Slm 6	N-	129 ± 1	187 ± 8	82 ± 1	115 ± 6	76 ± 1

Average productivities are calculated as the amount of TFA formed (mg) over the course of the experiment (12 days) per litre culture medium. Maximal productivities represent the highest productivity values between two consecutive time points. Average productivities are calculated for biomass, TFA and TAG. Maximal productivities are calculated for biomass and TFAs. Bold numbers represent the highest values for each parameter. ^aHighest value for nitrogen-replete cultivated strains; ^bhighest value for nitrogen-depleted cultivated cultures. Average values of biological duplicate cultures are shown followed by the deviation of the duplicates from the average. slm, starchless mutant; TAG, triacylglycerol; TFA, total fatty acid.

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