Figure 2
From: Improved production of fatty alcohols in cyanobacteria by metabolic engineering
Expression of the maqu_2220 gene and disruption of the sll0208 / sll0209 gene in Synechocystis. (A) Schematic representation of recombination to integrate the maqu_2220 gene expression cassette into slr0208 locus. slr0168-5' (or slr0168-3'), the 5' (or 3') end of slr0168 that was used for integrating the expressing cassette into the slr0168 locus by double homologous recombination. Omega, spectinomycin resistance gene. (B) Disruption of sll0208 in Synechocystis. sll0208-5' (or sll0208-3'), the 5' (or 3') end of sll0208 gene used for integrating the kanamycin resistance gene into the sll0208 locus by double homologous recombination. C.K2, the gene cassette containing a kanamycin resistance gene. (C) Disruption of both sll0208 and sll0209 in Synechocystis. The 5' end of sll0208 and the 3' end of sll0209 (sll0209-3') were used for integrating the kanamycin resistance gene into the sll0208 and the sll0209 locus by double homologous recombination. C.K2, the gene cassette containing a kanamycin resistance gene. PpetE, the promoter of the petE gene.