Figure 1From: Systematically programmed adaptive evolution reveals potential role of carbon and nitrogen pathways during lipid accumulation in Chlamydomonas reinhardtiiTwo dimensional dot plots (a, c) and flow cytograms (b, d) of BODIPY 505/515-stained 0 hour seed cells of CC124 (a, b) and sta6-1 (c, d). Region R2 (green), R3 (blue), and R4 (red) in flow cytograms represent low, medium, and high lipid content cells, respectively. A total of 25,000 cells from the top 2 to 3% of R4 region were sorted and regenerated in nitrogen depletion and repletion TAP medium, separately. The mean values of the histograms of the seed cells of CC124 and sta6-1 were 662.11 and 494.35, respectively. Abbreviations: BODIPY, 4,4-difluoro-1,3,5,7-tetramethyl-r-bora-3a,41-diaza-s-indacene.Back to article page