Figure 4

Analysis of the DNA-binding ability of the CREI-96 protein. (a) The EMSA was performed with a fluorescently labelled cbh1 URR fragment (4 ng) bearing three functional CREI-sites (red bars). (b) Applied were increasing amounts of the heterologously expressed and thrombin-cleaved CREI-96 protein (500, 1000, and 2000 nM), and 2000 nM of CREI-96 together with increasing amounts (50-fold, 100-fold, and 200-fold) of a cold competitor (unlabeled probe), and 2000 nM CREI-96 together with increasing amounts (50-fold, 100-fold, and 200-fold) of a mutated cold competitor. FP, free probe.