Figure 5

TAG production from mixed substrates of glucose, xylose, and glycerol by R. opacus MITGM-173. (a-c) Time course kinetics of TAG production as fatty acids. The strain was grown in modified defined media supplemented with 5.56 g L⁻¹ (NH₄)₂SO₄ containing a mixture of 40 g L⁻¹ xylose and 40 g L⁻¹ glucose (a), a mixture of 40 g L⁻¹ xylose, 40 g L⁻¹ glucose and 20 g L⁻¹ glycerol (b), and a mixture of 40 g L⁻¹ xylose and 40 g L⁻¹ glucose with pulse loading of 20 g L⁻¹ glycerol after 2 days of cultivation (c) in bioreactors. Values and error bars represent the mean and s.d. of triplicate experiments. (d) Thin-layer chromatography analysis of the crude organic extracts from the cells growing on xylose/glucose (a), xylose/glucose/glycerol (b), and xylose/glucose with pulse loading of glycerol (c) for 7 days. Lanes: 1, crude lipid extract (10 μg) on xylose/glucose; 2, crude lipid extract (10 μg) on xylose/glucose/glycerol; 3, crude lipid extract (10 μg) on xylose/glucose with pulse loading of glycerol; S, TAG (6 μg)/DAG (3 μg)/MAG (3 μg) mixtures. (e) Fatty acid composition as percentage of total fatty acids (g g⁻¹) of lipids from the cells growing in the defined medium containing xylose/glucose (a), xylose/glucose/glycerol (b), or xylose/glucose with pulse loading of glycerol (c) for 7 days. Data are results of triplicate experiments, ±s.d.