Figure 2

Spectra of the different coloured CPH substrates. (A) Product supernatants after a 1-h digestion of four differently coloured versions of CPH-HE cellulose with endo-cellulase (cel1) used at 2 U/mL in 100 mM sodium acetate buffer, pH 4.5 at room temperature. (B) Spectra of product supernatants shown in (A), together with the spectrum for the product of AZCL-hydroxyethyl-cellulose (Megazyme, black line), also digested with the same enzyme at 2 U/mL in 100 mM sodium acetate buffer, pH 4.5 for 1 h at room temperature. (C) and (D) Spectral response of colour combinations of differently coloured CPH-xylan mixed in a range of 100:0 to 0:100 with 10% increments (C) red and blue and (D) yellow and blue. See Tables 1 and 2 for details of the substrates and enzymes used.