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Table 2 Enzymes used in the study

From: A new generation of versatile chromogenic substrates for high-throughput analysis of biomass-degrading enzymes

Code name

Description

CAZy family

Source

ara

Endo-arabinase (Aspergillus niger)

GH43

Megazyme

cel1

Endo-cellulase (endo-β-1,4-glucanase) (Trichoderma longibrachiatum)

GH7

Megazyme

cel2

Cellulase (endo-β-1,4-glucanase) (Bacillus amyloliquefaciens)

GH5

Megazyme

Gal

Endo-β-1,4-D-galactanase (Aspergillus niger)

GH53

Megazyme

glc

Endo-β-1,3-glucanase (Trichoderma sp.)

GH16

Megazyme

lic

Lichenase (endo-β-1,3(4)-glucanase) (Bacillius sp.)

GH16

Megazyme

man

Endo-β-1,4-mannanase (Cellvibrio japonicus)

GH26

Megazyme

nz1

Endo-β-1,4-xylanase (Aspergillus aculeatus)

GH10

Novozymes

nz2

Endo-β-1,4-xylanase (Thermomyces lanuginosus)

GH11

Novozymes

nz3

Endo-β-1,4-glucanase (Aspergillus aculeatus)

GH5

Novozymes

nz4

Proprietary fungal endo-β-1,4-mannanase

GH5

Novozymes

ply1

Macerase™ Pectinase (Rhizopus sp.)

N/A

Calbiochem

ply2

Pectolyase Y-23 (Aspergillus japonicus)

N/A

Duchefa Biochemie

ply3

Pectolyase (Aspergillus japonicus)

N/A

Sigma

pec1

Pectate lyase (Cellvibrio japonicus)

PL10

Megazyme

pec2

Pectate lyase (Aspergillus sp.)

N/A

Megazyme

pol1

Endo-polygalacturonase M2 (Aspergillus niger)

GH28

Megazyme

pol2

Endo-polygalacturonase M1 (Aspergillus niger)

GH28

Megazyme

rgh

Rhamnogalacturonan hydrolase (Aspergillus aculeatus)

GH28

Novozymes

xg

Xyloglucanase (Paenibacillus sp.)

GH5

Megazyme

xyl1

β-xylanase, M4 (Aspergillus niger)

GH11

Megazyme

xyl2

Endo-β-1,4-xylanase M1 (Trichoderma viride)

GH11

Megazyme

NcLPMO9C

Lytic polysaccharide monoxygenase (Neurospora crassa)

AA9

N/A

Broth

Culture broth from Phanerochaete chrysosporium (3d after inoculation)

N/A

N/A

Proteinase K

Proteinase K (Tritirachium album)

N/A

Sigma

Trypsin

Trypsin from bovine pancreas

N/A

Sigma

Elastase

Elastase from porcine pancreas

N/A

Sigma

  1. Code, source and carbohydrate-active enzyme (CAZy) database family. N/A, not applicable.