Fig. 3From: Combinatory strategy for characterizing and understanding the ethanol synthesis pathway in cyanobacteria cell factoriesGenetic engineering of Synechocystis for regulation of PDCzm-slr1192 ratios. a Genetic modifications on genomes of Syn-ZG25, Syn-HZ24, and Syn-YQ4. Slr0168-UP and slr0168-Down up-stream and down-stream homologous regions for PrbcL-PDCzm-slr1192 cassette integration with slr0168 site. Slr9394-Up and slr9394-Down up-stream and down-stream homologous regions for PrbcL-PDCzm-slr1192 cassette integration with slr1993-slr1994 site. SpR spectinomycin resistance gene. KmR kanamycin resistance gene. b Western blot analysis for PDCzm and slr1192 concentration in PCC6803, Syn-ZG25, Syn-HZ24, and Syn-YQ4. c Pyruvate decarboxylase, alcohol dehydrogenase, and PDCzm-slr1192 pathways activities calculations for crude enzyme of Syn-ZG25, Syn-HZ24, and Syn-YQ4Back to article page