Table 2 2-oxo acid decarboxylase activities in cell extracts of S. cerevisiae strains expressing single 2-oxo acid decarboxylases, grown on different nitrogen sources
Substrate | Strain | Nitrogen source | ||
|---|---|---|---|---|
NH4 + | Phenylalanine | Valine | ||
Pyruvate | IME259 (control) | BD | NG | NG |
IME260 (ARO10↑) | BD | BD | NG | |
IME261 (kdcA↑) | 0.013 ± 0.001 | 0.036 ± 0.006 | 0.033 ± 0.005 | |
IME262 (kivD↑) | BD | 0.012 ± 0.003 | BD | |
Phenylpyruvate | IME259 (control) | BD | NG | NG |
IME260 (ARO10↑) | BD | 0.046 ± 0.001 | NG | |
IME261 (kdcA↑) | 0.045 ± 0.008 | 0.184 ± 0.029 | 0.155 ± 0.037 | |
IME262 (kivD↑) | BD | 0.385 ± 0.040 | 0.013 ± 0.000 | |
α-ketoisovalerate | IME259 (control) | BD | NG | NG |
IME260 (ARO10↑) | BD | 0.056 ± 0.001 | NG | |
IME261 (kdcA↑) | 0.683 ± 0.146 | 2.509 ± 0.509 | 1.900 ± 0.153 | |
IME262 (kivD↑) | 0.093 ± 0.016 | 2.885 ± 0.107 | 0.151 ± 0.030 | |