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Table 4 Estimated in vivo activities of 2-oxo acid decarboxylases during α-ketoisovalerate (KIV) bioconversion experiments

From: Comparative assessment of native and heterologous 2-oxo acid decarboxylases for application in isobutanol production by Saccharomyces cerevisiae

Strain

Decarboxylase

Substrate

Pyruvate

KIV

IME259

None

BD

BD

IME260

ARO10↑

0.71 ± 0.29

2.02 ± 0.02

IME261

kdcA↑

8.07 ± 1.29

4.05 ± 0.05

IME262

kivD↑

3.74 ± 0.07

2.81 ± 0.38

IME140

Wild-type control

114 ± 3

3.83 ± 0.14

  1. Pyruvate and KIV decarboxylase activities were estimated from ethanol and isobutanol production rates at pH 3.5, based on a biomass protein content of 42 % [23]. Activity is expressed in mU mg protein−1
  2. BD below detection limit of HPLC
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Biotechnology for Biofuels and Bioproducts

ISSN: 2731-3654

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