Table 1 Sequence variants from sequenced wild-type Cpa 6013 vs. M150B

135360

Sub.

No CDS

A

G

177873

Sub.

Hypothetical cell wall-binding protein, c01710

A267V

C

T

256066

Sub.

Glycosyltransferase, c02430

I257T

A

G

328268

Sub.

Xanthine dehydrogenase, molybdenum binding; pudD1

P483L

C

T

511135

Sub.

Pyridine nucleotide-disulfide oxidoreductase; NADH dehydrogenase; coenzyme A disulfide reductase; c04890

P187S

T

C

547010

Sub.

Pyruvate-flavodoxin oxidoreductase; nifJ1

S579N+

G

A

609918

Sub.

Lactose permease; lacS

P429S

G

A

623217

Sub.

lysin; c05770

A129T

G

A

633860

Sub.

Hypothetical protein; c05850

Silent

C

T

662676

Sub.

High-affinity nickel-transport protein; nixA1

Silent

C

T

664502

Sub.

Methyl-accepting chemotaxis protein; c06130

T311A

A

G

774540

Sub.

Metallophosphoesterase; c07050

G294E

G

A

805746

Sub.

Putative amidase domain-containing protein; c07320

G378E

G

A

822080

Sub.

tRN Glu)-specific nuclease WapA; wapA1

S1026F

C

T

849186

Sub.

Putative glycosyltransferase; c07760

Q641tag

G

A

888969

Sub.

Phage infection protein; c08050

E95K+

C

T

905016

Sub.

putative L-ascorbate-6-phosphate lactonase UlaG; ulaG

A128V

C

T

959280

Sub.

No CDS

C

T

1010600

Sub.

Radical SAM domain protein; c09370

G32D

C

T

1018923

Sub.

No CDS

G

A

1034853

Sub.

Putative AAA-ATPase; c09620

E311K+

G

A

1047038

Sub.

DNA-binding response regulator; c09780

Silent

G

A

1052341

Sub.

Hypothetical protein; c09830

E98K+

G

A

1058795

Sub.

Nicotinamidase; c09830

V31I+

G

A

1263298

Sub.

No CDS

G

A

1384612

Sub.

Spore germination protein KA; gerKA2

G15D

G

A

1408727

Sub.

hypothetical protein; c13080

L71N

G

A

1595471

Sub.

Lactate-responsive regulator LldR; c14940

A154T

G

A

1597713

Sub.

Electron transfer flavoprotein, alpha subunit; etfA1

A300T

G

A

1608597

Sub.

CAAX amino terminal protease family protein; c15040

Silent

T

C

1729728

Sub.

Integral membrane protein; c16180

V8I+

G

A

1796914

Sub.

Phenylalanyl-tRNA synthetase beta chain; pheT

A674V

C

T

1957368

Sub.

Flagellum-specific ATP synthase FliI; fliI

E44K+

G

A

1973896

Sub.

RNA polymerase sigma factor for flagellar operon; whiG

A75T

G

A

1976216

Sub.

Flagellar basal-body rod protein; flgG3

Silent

T

C

2055679

Del.

Stage 0 sporulation protein A; spo0A

Del. 235–242

ATACCATAAATAAATTATTTGGAT

2822361

Sub.

CRISPR-associated helicase/endonuclease Cas3; cas3

E316F

C

T

3198907

Sub.

No CDS

C

T

3208358

Sub.

Exonuclease SbcC; sbcC

A558T

C

T

3222314

Sub.

Hypothetical protein, CF-17 family; c29890

E213K+

C

T

3270753

Sub.

No CDS

C

T

3272757

Sub.

tRNA-dihydrouridine synthase; c30410

D126K+

C

T

3273913

Sub.

Fe-S oxidoreductase; c30430

T87A

A

G

3297381

Sub.

No CDS

G

A

3348577

Sub.

Transcriptional regulator, TetR family; c31160

S20N+

C

T

3399757

Sub.

Hydrolase (HAD superfamily); c31590

P219S

G

A

3439637

Sub.

Multiple sugar transporter, membrane-spanning permease protein MsmG; msmG2

A236V

G

A

3488562

Sub.

Potassium-transporting ATPase C chain; kdpC

T283I

G

A

3497476

Sub.

Fatty-acid peroxygenase; cypC

P255S

G

A

3521979

Sub.

ATP phosphoribosyltransferase regulatory subunit; hisZ

P96L

G

A

3531067

Sub.

Cysteine desulfurase; c32790

Silent

G

A

3535341

Sub.

Chaperone protein HtpG; htpG

Silent

G

A

3593919

Sub.

No CDS

G

A

3595710

Sub.

2-iminoacetate synthase; thiH2

P77S

G

A

3641811

Sub.

Possible surface protein; c33730

T742I

G

A

3666696

Sub.

No CDS

G

A

3698846

Sub.

L-arabinose transport system permease; araQ2

T235I

G

A

3759007

Sub.

hypothetical protein; c34910

E729K+

C

T

3776313

Sub.

RNA-binding protein; c35120

S523N+

C

T

3863797

Sub.

DNA-3-methyladenine glycosylase II; c35950

E119K+

C

T

3872706

Sub.

Autoinducer 2 sensor kinase/phosphatase LuxQ; luxQ3

E242F

C

T

3909641

Sub.

Signal-transduction and transcriptional-control protein; stc3

A657V+

C

T

3935380

Sub.

50S ribosomal protein L2; rplB

Silent

C

T

3936650

Sub.

50S ribosomal protein L3; rplC

G191D

C

T

4163498

Sub.

Signal transduction histidine kinase; c39070

S439N

C

T

4231311

Sub.

Putative competence-damage inducible protein; cinA

Silent

C

T