Fig. 2

Carbohydrate solubilization from washed mid-season switchgrass by various enzyme loadings. C. thermocellum enzymes (blue) were purified by either affinity purification filled circle or by concentrating and dialyzing cell-free broth circle. Fungal cellulase (red) was incubated at 37C in the presence (filled diamond) or absence of yeast (open diamond), at lower substrate concentration (X, 1 g/L glucan, 2.5 g/L solids), or increased hydrolysis temperature (square, 50 °C). Results are after 5 days and are expressed as mean ± SD (n ≥ 2 except for dialyzed concentrated broth)