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Table 2 R-M systems in C. pasteurianum NRRL B-598 genome

From: Dam and Dcm methylations prevent gene transfer into Clostridium pasteurianum NRRL B-598: development of methods for electrotransformation, conjugation, and sonoporation

Type

Name

Genea

Meth. type

Recognition

Locus (X276_)

Most similar (% identity)

I

Cpa598IP

R

m6A

GAAYNNNNNNNRTANYC

10620

CspMORF4102P (95 %)

M.Cpa598I

M

10630

M.CbeG117ORFCP (97 %)

S.Cpa598I

S

10635

S.CspMORF4102P (49 %)

I

Cpa598IIP

R

m6A

GAYNNNNNNCTAG

12355

Csc25775ORFJP (90 %)

M.Cpa598II

M

12360

M.Csc25775ORFJP (96 %)

S.Cpa598II

S

12365

S.Bme201ORFGP (56 %)

II

M1.Cpa598ORF20205

M

m5C

–

–

M1.CboKAPB3ORF12160P (87 %)

M2.Cpa598ORF20205

M

01545

M2.CboKAPB3ORF12160P (84 %)

R1.Cpa598ORF20205

R

–

R2.Cce743ORF4007P (46 %)

R2.Cpa598ORF20205

R

01555

R1.Bce3081ORF2217P (31 %)

II

M.Cpa598ORF2410P

M

m6A

GATCb

20735

M.Cbe59BORF1284P (100 %)

IV

Cpa598ORF12465P

R

–

–

12465

Cdi15410ORFAP (93 %)

  1. a R restriction endonuclease, M restriction endonuclease coupled methylation protein, S R-M specific protein
  2. b Predicted recognition site
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Biotechnology for Biofuels and Bioproducts

ISSN: 2731-3654

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