Table 4 Kinetic parameters of Tyr305 variants for synthetic substrate
From: Directed evolution of a fungal β-glucosidase in Saccharomyces cerevisiae
app K m (mM) | app V max (μmole L−1 min−1) | app K i pNPG (mM) | |
|---|---|---|---|
Y305F | 0.46 ± 0.04 | 126.5 ± 5.4 | 4.78 ± 0.44 |
Y305Wa | NA | NA | NA |
Y305G | 1.16 ± 0.06 | 239.7 ± 3.9 | – |
Y305A | 1.55 ± 0.06 | 238.2 ± 4.1 | – |
Y305 V | 1.14 ± 0.08 | 233.9 ± 5.6 | – |