Penicillium echinulatum secretome analysis reveals the fungi potential for degradation of lignocellulosic biomass

Table 7 Identified starch-degradation enzymes and spectrum counts in different carbon sources by wild-type 2HH and mutant S1M29 of Penicillium echinulatum at 96 h during submerged cultivation

Accession number	Identified proteins	CAZyme	Organism	MW (kDa)	Secretion^b	Spectrum count^a
						2HH				S1M29
						Glu	Gly	SCB	Cel	Glu	Gly	SCB	Cel
g4201	α-amylase	GH13	Penicillium oxalicum	74	Y	0	2	2	1	0	1	0	6
g5478	Glucoamylase Amy15A	GH15 (CBM20)	Penicillium oxalicum	66	Y	1	1	0	0	9	10	0	0
g36	Glucoamylase	GH15 (CBM20)	Penicillium oxalicum	68	Y	1	0	0	0	14	0	0	0
g7292	α-glucosidase	GH31	Penicillium oxalicum	108	Y	0	7	0	0	0	1	0	0
g1651	α-trehalase	GH65	Penicillium oxalicum	119	Y	0	0	0	0	0	23	0	0

^aSecretomic analysis based on spectral counting. A quantitative analysis was conducted for samples grown on cellulose or SCB (mean of triplicates), while a semiquantitative analysis for samples grown on glucose or glycerol (one replicate) was performed. The complete protein reports are given in Additional file 3
^bThe secretion of each protein was verified by the softwares SignalP, SecretomeP and YLoc. When at least two of three softwares give a positive result the protein was considered secreted

ISSN: 2731-3654