Inhibition of lignin-derived phenolic compounds to cellulase

Table 1 Effect of additives on cellulose conversion^a and apparent inhibition degrees

Additives	Cellulose conversion without vanillin (%)	Cellulose conversion with vanillin (%)	Apparent inhibition (%)
Blank^b	41.9 ± 0.5	26.8 ± 0.6	35.9 ± 0.6
CaCl ^b₂	42.8 ± 0.2	27.0 ± 1.3	36.7 ± 0.5
Tween 80^b	42.0 ± 1.5	28.5 ± 1.5	32.2 ± 1.5
Granular activated carbon^b	39.7 ± 0.8	24.8 ± 1.4	37.5 ± 1.1
Powdered activated carbon^b	28.6 ± 0.6	14.1 ± 1.7	50.5 ± 1.0
BSA^b	42.0 ± 1.0	27.5 ± 0.3	34.6 ± 0.6
Blank^c	41.9 ± 0.5	32.4 ± 1.0	22.6 ± 0.3
BSA^c	40.8 ± 0.3	31.5 ± 0.3	22.8 ± 0.3
BSA^{c, d}	40.8 ± 0.8	31.5 ± 1.0	23.2 ± 0.9

^aEnzymatic hydrolysis of Avicel was carried out with cellulose concentration of 10 mg/mL and cellulase concentration of 0.3 mg/mL at 50 ºC. Cellulose conversion was determined at 24 h. The concentration of the additives was 2.5 mg/mL
^bVanillin concentration was 5 mg/mL
^cVanillin concentration was 2.5 mg/mL
^dVanillin was pre-incubated 3 h with BSA before enzyme addition

ISSN: 2731-3654