Table 1 Relative lipid contents of strains with overexpressed or deleted genetic targets in addition to DGA1 overexpression
From: Engineering of a high lipid producing Yarrowia lipolytica strain
Genetic background | Function | (Fl/OD)mutant/(Fl/OD)NS125 | |
|---|---|---|---|
WT | Wild-type Yarrowia lipolytica strain | 0.55 ± 0.02 | |
DGA1 (NS125) | Diacylglycerol acyltransferase; catalyzes the terminal step of triacylglycerol (TAG) formation, acylates diacylglycerol using acyl-CoA as an acyl donor | 1.00 ± 0.09 | |
DGA1 | Δgut2 | Mitochondrial glycerol-3-phosphate dehydrogenase | 0.95 ± 0.12 |
DGA1 | GPD1 | NAD-dependent glycerol-3-phosphate dehydrogenase | 1.12 ± 0.03 |
DGA1 | ME | Mitochondrial malic enzyme; catalyzes the oxidative decarboxylation of malate to pyruvate | 0.92 ± 0.15 |
DGA1 | ACL1/2 | ATP citrate lyase 1 & 2 | 0.99 ± 0.09 |
DGA1 | ACC1 | Acetyl-CoA carboxylase subunit 1 | 1.05 ± 0.19 |
DGA1 | SCT1 | Bifunctional glycerol-3-phosphate/glycerone-phosphate O-acyltransferase | 1.03 ± 0.01 |
DGA1 | SLC1 | 1-acylglycerol-3-phosphate O-acyltransferase | 1.25 ± 0.07 |
DGA1 | SCD1 | Stearoyl-CoA desaturase. Endoplasmic reticulum (ER) protein that catalyzes the Δ9-cis desaturation of saturated fatty acids | 0.85 ± 0.18 |
DGA1 | DGA2 | DGAT1 acyl-CoA:diacylglycerol acyltransferase family | 1.62 ± 0.03 |
DGA1 | LRO1 | Acyltransferase that catalyzes diacylglycerol esterification; one of several acyltransferases that contribute to triglyceride synthesis | 1.33 ± 0.07 |
DGA1 | Δtgl3 | Bifunctional triacylglycerol lipase. Major lipid particle-localized triacylglycerol (TAG) lipase | 0.79 ± 0.24 |
DGA1 | Δtgl4 | Multifunctional lipase/hydrolase/phospholipase; triacylglycerol lipase, steryl ester hydrolase, and Ca2 + -independent phospholipase A2 | 1.03 ± 0.08 |
DGA1 | Δmfe1 | Multifunctional enzyme, member of the peroxisomal hydroxyacyl coenzyme A dehydrogenase family | 0.89 ± 0.03 |