Fig. 1

Schematic illustrating the mobilization of Y. lipolytica metabolism for fatty alcohol production. Fatty alcohol accumulation was attempted by introducing fatty acyl-CoA reductase (FAR) and eliminating degradation pathways involving fatty alcohol oxidase (FAO), alcohol dehydrogenase (ADH), and fatty alcohol dehydrogenase (FADH). Further improvement of fatty alcohol production was tried by increasing fatty acyl-CoA supply: knock-out of genes responsible for fatty acyl-CoA degradation (transporter PXA and peroxisome biogenesis-involved PEX10) and conversion from fatty acyl-CoA to TAG (acyl-CoA:diacylglycerol acyltransferase DGA and phospholipid:diacylglycerol acyltransferase LRO) or sterol (ACAT-related sterol acyl-CoA acyltransferase (SAT) isozyme ARE); overexpression of genes responsible for directing metabolic flux to fatty acyl-CoA (ATP-citrate lyase ACL, acetyl-CoA synthetase ACS, acetyl-CoA carboxylase ACC, and fatty acyl-CoA synthetase FAA)