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Table 4 Raw sugar beet juice wort before and after fermentation

From: Ozonation as an effective way to stabilize new kinds of fermentation media used in biotechnological production of liquid fuel additives

Physicochemical parameters

Wort before fermentation

Control sample A (fresh raw juice without treatment, the initial total count of bacteria 2.5 × 103 cfu/mL)

Control sample B (raw juice without treatment, initial total count of bacteria 2.0 × 105 cfu/mL)

Sample B after

Sterilization

Ozonation

Dry matter (g kg−1)

146.0 a ± 5.0

145.0 a ± 6.0

145.0 a ± 6.0

138.0 a ± 4.0

pH

4.8 a ± 0.2

4.8 a ± 0.1

4.8 a ± 0.1

4.8 a ± 0.1

Sugars

Reducing (g inverted sugar/kg)

9.4 a ± 0.3

9.7 a ± 0.3

9.3 a ± 0.4

11.7 b ± 0.4

Saccharose (g/kg)

113.7 b ± 3.4

112.5 b ± 2.4

113.1 b ± 4.5

94.8 a ± 3.8

Total (g inverted sugar/kg)

129.1 b ± 3.9

128.1 b ± 3.8

128.4 b ± 5.1

111.5 a ± 4.5

Physicochemical parameters

Wort after fermentation

Control sample A (fresh raw juice without treatment, initial total count of bacteria 2.5 × 103 cfu/mL)

Control sample B (raw juice without treatment, the initial total count of bacteria 2.0 × 105 cfu/mL)

Sample B after

Sterilization

Ozonation

Dry matter (g/kg)

26.5 a ± 2.0

26.2 a ± 1.5

28.2 a ± 2.1

26.1 a ± 1.0

pH

3.9 a ± 0.1

3.4 b ± 0.3

3.8 a ± 0.1

3.8 a ± 0.1

Sugars

Reducing (g inverted sugar/kg)

1.9 b ± 0.1

1.4 a ± 0.1

1.6 a ± 0.1

1.9 b ± 0.2

Saccharose (g/kg)

0.5 b ± 0.1

0.3 a ± 0.1

0.2 a ± 0.1

0.6 c ± 0.0

Total (g inverted sugar/kg)

2.4 b ± 0.1

1.9 a ± 0.1

1.9 a ± 0.1

2.5 b ± 0.1

Sugar consumption (%)

98.14 a ± 2.90

98.80 a ± 2.50

98.52 a ± 3.00

98.22 a ± 2.80

Ethanol concentration (% v/v)

7.53 a ± 0.30

6.25 b ± 0.25

7.56 a ± 0.29

7.03 a ± 0.27

Yield of ethanol (% of theoretical yield)

90.07 a ± 2.80

75.30 b ± 3.00

90.87 a ± 2.70

97.37 b ± 3.80

  1. All values are means of triplicate measurements ± standard deviation (SD)
  2. a, b, c—means in rows with different letters differ significantly at p < 0.05