The micromorphology of Trichoderma reesei analyzed in cultivations on lactose and solid lignocellulosic substrate, and its relationship with cellulase production

Table 1 Fungal micromorphology, protein concentration and cellulase activity obtained in cultivations on lactose and wheat straw by T. reesei strains Δcre1 and QM9414

	Cell length (µm)	Cell width (µm)	Branches/cell (-)	FPU/mL	Protein (g/L)
T. reesei Δcre1
Wheat straw
Agar-190	10.21 ± 3.43	3.29 ± 1.34	0.40	1.74	0.59
Agar-150	10.08 ± 3.17	2.80 ± 0.80	0.41	0.69	0.33
Spore-190	8.82 ± 4.18	3.85 ± 0.89	0.38	1.88	0.57
Spore-150	9.75 ± 2.92	3.47 ± 1.06	0.48	1.82	0.30
Agar-190-Tw	11.73 ± 3.81	3.60 ± 1.51	0.40	1.27	0.38
Spore-190-Tw	7.87 ± 2.21	3.98 ± 1.09	0.08	2.31	0.59
Average value	9.74 ± 1.32	3.50 ± 0.42	0.36 ± 0.14	n.c.	n.c.
Lactose
Agar-190	11.75 ± 4.12	3.01 ± 0.77	0.43	n.d.	0.27
Agar-150	11.67 ± 4.64	3.20 ± 0.56	0.25	n.d.	0.26
Spore-190	10.51 ± 3.20	3.42 ± 0.97	0.28	n.d.	0.30
Spore-150	8.55 ± 3.32	2.91 ± 1.01	0.50	n.d.	0.26
Average value	10.62 ± 1.49	3.13 ± 0.23	0.36 ± 0.12	n.c.	n.c.
T. reesei QM9414
Wheat straw
Agar-190	11.84 ± 3.43	3.05 ± 0.94	0.05	0.88	0.64
Agar-150	10.21 ± 2.92	3.04 ± 1.03	0.40	0.84	0.31
Spore-190	10.42 ± 3.15	3.32 ± 1.23	0.01	1.45	0.58
Spore-150	10.20 ± 3.21	2.35 ± 0.95	0.26	1.64	0.26
Agar-190-Tw	12.47 ± 3.83	2.67 ± 1.27	0.06	0.77	0.43
Spore-190-Tw	10.09 ± 2.67	3.30 ± 1.41	0.40	1.14	0.44
Average value	10.87 ± 1.02	2.95 ± 0.38	0.16 ± 0.15	n.c.	n.c.
Lactose
Agar-190	16.74 ± 5.40	2.59 ± 0.69	0.25	n.d.	0.09
Agar-150	14.24 ± 4.11	3.03 ± 1.20	0.31	n.d.	0.25
Spore-190	13.53 ± 4.82	2.82 ± 0.64	0.23	n.d.	0.09
Spore-150	10.59 ± 3.47	3.32 ± 0.53	0.49	n.d.	0.26
Average value	13.77 ± 2.19	2.94 ± 0.31	0.32 ± 0.09	n.c.	n.c.

Volumetric cellulase activity (FPU/mL) and protein concentration represent mean values of two experiments. Dimensions of the cells represent mean values and the spread from 40 cells analyzed from one representative CLSM image. The branches per cell were analyzed in the same image and represent the value of 60–100 cells
n.c. not calculated, n.d. not detectable

ISSN: 2731-3654