Table 2 Effects of metal ions and chemicals on DT-Bgl activity
From: Characterization of a glucose-tolerant β-glucosidase from Anoxybacillus sp. DT3-1
Relative activity (%) | Relative activity (%) | ||||
|---|---|---|---|---|---|
2 mM | 5 mM | 1 % (v/v) | 5 % (v/v) | ||
Control | 100.00 ± 0.01 | 100.00 ± 0.01 | Control | 100.00 ± 0.01 | 100.00 ± 0.01 |
CaCl2 | 79.33 ± 0.02 | 70.58 ± 0.01 | Tween-20 | 91.62 ± 0.02 | 1.61 ± 0.00 |
NaCl | 96.08 ± 0.01 | 91.13 ± 0.03 | Tween-40 | 106.40 ± 0.01 | 0.67 ± 0.00 |
KCl | 96.14 ± 0.01 | 94.19 ± 0.02 | Tween-80 | 61.98 ± 0.01 | 0.25 ± 0.01 |
MgCl2 | 84.48 ± 0.01 | 80.17 ± 0.01 | Triton X-100 | 99.26 ± 0.01 | 90.01 ± 0.02 |
FeCl3 | 7.07 ± 0.04 | 0.03 ± 0.00 | DMSO | 104.20 ± 0.01 | 92.29 ± 0.03 |
NiCl2 | 68.06 ± 0.00 | 3.56 ± 0.00 | |||
CoCl2 | 87.69 ± 0.00 | 9.32 ± 0.01 | |||
NH4Cl | 91.79 ± 0.03 | 88.18 ± 0.01 | |||
ZnCl2 | 10.21 ± 0.00 | 2.27 ± 0.00 | |||
MnCl2 | 46.38 ± 0.04 | 34.81 ± 0.00 | |||
Urea | 100.62 ± 0.01 | 98.59 ± 0.01 | |||
SDS | 72.39 ± 0.03 | 16.70 ± 0.01 | |||
EDTA | 90.53 ± 0.02 | 89.94 ± 0.00 | |||