Fig. 10

Effect of SWO1 supplementation on the enzymatic hydrolysis of cock’s-foot grass. A Reactions were done in 50 mM sodium acetate buffer, pH 5.0, at 40 °C with shaking (500 rpm) in a total reaction volume of 1 mL over 164 h. Substrate concentration was 5.0 mg/mL, and cellulase was added to a final protein loading of 2 µg/mg substrate. SWO1 was present at 0.02 µM (black bar), and the reference experiment used an equimolar amount of BSA (grey bar) instead of SWO1. The amount of reducing sugars released was measured colorimetrically with the 3,5-DNS assay calibrated against glucose. Error bars were estimated from two independent experiments. B By comparison with the BSA-containing control reaction (left panel), cock’s-foot grass appeared to be more completely degraded in the presence of SWO1 (central panel) after 164 h. The remaining substrate parts are highlighted for an easier viewing. The untreated material is shown as reference (right panel)