Fig. 4

Activity of SWO1 on various glycan substrates. a The substrates used were Avicel PH-101, CNC and β-glucan (1 mg/mL each) in 50 mM sodium acetate buffer, pH 5.0. Incubation was for 24 h at 40 °C with shaking (500 rpm). Avicel PH-101 and CNC were incubated with 0.4 µM SWO1 (black bars) or an equimolar amount of BSA (grey bars). Reactions were stopped by heating and incubated with β-glucosidase. The glucose released was measured with an enzymatic assay. Error bars show SD from four independent experiments. Barley β-glucan was incubated with either 0.2 µM SWO1 or BSA. The liberated sugars were assayed with HPAEC-PAD, and cellobiose was identified as the main product of SWO1 activity. Error bars are from two independent experiments. b Cellotetraose (0.5 mg/mL) was incubated with either 0.5 µM SWO1 or BSA in 50 mM sodium acetate buffer, pH 5.0, for 24 h at 40 °C with shaking (500 rpm). The product distribution (G2 cellobiose, G3 cellotriose, G4 cellotetraose) was determined with HPAEC-PAD. Error bars were estimated from two independent experiments