Skip to main content

Table 3 Selected genes overexpressed in performing inocula

From: Phenotypic characterization and comparative transcriptomics of evolved Saccharomyces cerevisiae strains with improved tolerance to lignocellulosic derived inhibitors

Gene

Description

FCa

Fatty acid metabolism

 TES1

Peroxisomal acyl-CoA thioesterase, involved in fatty acid oxidation

5.0

 PXA2

Peroxisomal ATP-binding cassette transporter

2.7

 OLE1

Fatty acid desaturase, required for proper mitochondrial functioning

2.6

 ETR1

Thioester reductase, required for proper mitochondrial functioning

2.2

General cellular metabolism

 TDH3

Glyceraldehyde-3-phosphate dehydrogenase

8.2

 DFR1

Dihydrofolate reductase, involved in tetrahydrofolate synthesis

4.3

 ALD3/ALD2

Aldehyde dehydrogenase

4.2

 PDC6/PDC1

Pyruvate decarboxylase

4.0

 FDH1

Formate dehydrogenase

3.8

 TKL1/TKL2

Transketolase

3.6

 ASN1

Asparagine synthetase

3.1

 ARO80

Transcriptional activator for aromatic amino acid catabolism.

2.9

 ADH1

Alcohol dehydrogenase

2.9

Membrane/cell wall associated

 PUN1

Plasma membrane protein

5.2

 HES1

Implied regulator of ergosterol synthesis

4.3

 PIR1

Glycosylated cell wall protein, required for Apn1p mitochondrial translocation

2.3

Transport

 SEC27

Protein in the COPI coatomer

4.6

 ATG20

Sorting nexin required for cytoplasm to vacuole targeting

4.4

 CHS5

Exomer complex component

4.4

 CCC2

Copper transporting P-type ATPase

3.5

 FLC1

Putative FAD transporter

3.4

 PCA1

Cadmium transporting P-type ATPase

2.9

 SUL1

High affinity sulfate permease

2.9

 SEC22

R-SNARE protein

2.6

 GYP7

GTPase-activating protein

2.6

 PDR10

ATP-binding cassette (ABC) transporter, multidrug transporter

2.6

 SGE1

Plasma membrane multidrug transporter

1.9

Mitochondria associated

 MRM2

Mitochondrial O-ribose methyltransferase

4.6

 YNL195C

Unknown function, part of the mitochondrial proteome

4.1

 RPO41

Mitochondrial RNA polymerase

3.7

 FMP48

Unknown function, part of the mitochondrial proteome

3.7

 MSH1

MutS Homologue involved in mitochondrial DNA repair

3.4

 AEP2

Mitochondrial protein involved in translation of OLI1 mRNA

3.4

 YKL187C

Unknown function, part of the mitochondrial proteome

2.8

 YNL208W

Unknown function, part of the mitochondrial proteome

2.8

 MKS1

Pleiotropic transcriptional regulator, involved in retrograde signaling

2.7

 APJ1

Chaperone protein involved in SUMO-mediated protein degradation

2.7

 MIP1

Mitochondrial DNA polymerase

2.6

 MSC1

Unknown function, part of the mitochondrial proteome

2.5

 PUT1

Proline oxidase

2.5

 FMP16

Unknown function, part of the mitochondrial proteome

2.5

 CYB2

Cytochrome b2

2.3

 YNL200C

NADHX epimerase

2.0

 DLD3

d-Lactate dehydrogenase, part of the retrograde regulon

2.0

DNA stress response

 IWR1

RNA Pol II transport factor, relocates to cytoplasm upon DNA stress

4.4

 ENO1

Enolase I, converts 2-phosphoglycerate to phosphoenolpyruvate

2.7

 TFS1

Inhibits carboxypeptidase Y and Ira2p

2.4

 TPS2

Phosphatase involved in synthesis of trehalose

2.0

Oxidative stress response

 CTT1

Cytosolic catalase T

3.7

 GTT1

Glutathione transferase

2.7

 GAD1

Glutamate decarboxylase

2.1

 AHP1

Thiol-specific peroxiredoxin

2.1

  1. aFold change, average expression of performing inocula compared to nonperforming