Fig. 4From: Improvement of free fatty acid production using a mutant acyl-CoA thioesterase I with high specific activity in Escherichia coli Free fatty acid production from ‘TesA mutants at position 64 generated by site-directed mutagenesis. The expression of ‘TesA was induced with 0.3 mM IPTG, and the cells were cultivated for 48 h post-induction. Parenthesises indicate mutation of the ‘TesA. Filled circles indicate the optical density of each culture after 48 h. Error bars mean standard deviations of three independent experimentsBack to article page