Fig. 5From: Improvement of free fatty acid production using a mutant acyl-CoA thioesterase I with high specific activity in Escherichia coli Kinetic analysis of the ‘TesA (filled circle) and ‘TesAR64C (filled square). The values are average of three independent experiments. The kinetic parameters were calculated by nonlinear repression plots of the Michaelis–Menten equation. The enzyme concentration was 3.1 × 10−5 mMBack to article page