Table 1 Strains and plasmids used in this study
Strains and plasmids | Description | Reference |
|---|---|---|
Strains | ||
 Candida tropicalis ATCC20336 | Source for CtPDC1 | In this study |
 Kluyveromyces marxianus KCTC17555 | Source for KmPDC1 | In this study |
 Saccharomyces cerevisiae D452-2 | Source for ScPDC1, ScPDC5, ScPDC6 MATα leu2 his3 ura3 | [49] |
 BD4 | D452-2, pdc1Δ, pdc5Δ, evolved 2,3-BD-producing strain | [12] |
 SOS5 | D452-2, pdc1Δ, pdc5Δ, pdc6Δ | [18] |
 SOS5_T2CtPDC1 | SOS5, p426TDH3_CtPDC1 | In this study |
 SOS5_T2KmPDC1 | SOS5, p426TDH3_KmPDC1 | In this study |
 SOS5_T2ScPDC1 | SOS5, p426TDH3_ScPDC1 | In this study |
 SOS5_T2ScPDC5 | SOS5, p426TDH3_ScPDC5 | In this study |
 SOS5_T2ScPDC6 | SOS5, p426TDH3_ScPDC6 | In this study |
 BD5 | SOS5, p423_alsSalsD, p425_BDH1 | [18] |
 BD5_Con | BD5, p426GPD | [18] |
 BD5_C1CtPDC1 | BD5, p406CYC1_CtPDC1 | In this study |
 BD5_G1CtPDC1 | BD5, p406GPD2_CtPDC1 | In this study |
 BD5_C2CtPDC1 | BD5, p426CYC1_CtPDC1 | In this study |
 BD5_T2CtPDC1 | BD5, p426TDH3_CtPDC1 | In this study |
 BD5_G1CtPDC1_nox | BD5, p406GPD2_CtPDC1, pAUR_Llnox | In this study |
Plasmids | ||
 pRS406 | URA3 | [50] |
 pRS426 | URA3 2 μm origin | [50] |
 p406GPD2 | pRS406, GPD2 prom CYC1 term | [18] |
 p426CYC1 | pRS426, CYC1 prom CYC1 term | [18] |
 p426GPD | URA3 2 μm origin, TDH3 prom CYC1 term | [50] |
 p423_alsSalsD | HIS3 2 μm origin, TDH3 prom-alsS-CYC1 term TDH3 prom-alsD-CYC1 term | [51] |
 p425_BDH1 | LEU2 2 μm origin, TDH3 prom-BDH1-CYC1 term | [12] |
 p426TDH3_Llnox | pRS426, TDH3 prom-Llnox-CYC1 term | [18] |
 p426TDH3_CtPDC1 | pRS426, TDH3 prom-CtPDC1-CYC1 term | In this study |
 p426TDH3_KmPDC1 | pRS426, TDH3 prom-KmPDC1-CYC1 term | In this study |
 p426TDH3_ScPDC1 | pRS426, TDH3 prom-ScPDC1-CYC1 term | In this study |
 p426TDH3_ScPDC5 | pRS426, TDH3 prom-ScPDC5-CYC1 term | In this study |
 p426TDH3_ScPDC6 | pRS426, TDH3 prom-ScPDC6-CYC1 term | In this study |
 p406CYC1_CtPDC1 | pRS406, CYC1 prom-CtPDC1-CYC1 term | In this study |
 p406GPD2_CtPDC1 | pRS406, GPD2 prom-CtPDC1-CYC1 term | In this study |
 p426CYC1_CtPDC1 | pRS426, CYC1 prom-CtPDC1-CYC1 term | In this study |
 pAUR_Llnox | AUR1-C CEN6 ARS4, TDH3 prom-Llnox-CYC1 term | In this study |