Fig. 5
From: Self-regulated 1-butanol production in Escherichia coli based on the endogenous fermentative control
Effect of oxygen level on the FRE-controlled 1-butanol fermentation. Strain ΔldhA ΔfrdBC ΔadhE Δpta transformed with the best combination (FRE ackA ::atoB-adhE2-crt-hbd, FRE adhE ::ter, FRE adhE ::fdh) was used in this study. Cells were grown in TB medium aerobically to mid-log phase then switched into various cultivation vessels to simulate the different oxygen levels as described in “Methods” section. Samples were taken after 24 h of anaerobic switch. GLC, glucose