Table 1 Strains and plasmids used in this study
From: Self-regulated 1-butanol production in Escherichia coli based on the endogenous fermentative control
| Â | Genotype | Reference |
|---|---|---|
Strain | ||
BW25113 | rrnB T14 ∆lacZ WJ16 hsdR514 ∆araBAD AH33 ∆rhaBAD LD78 | [45] |
XL-1 blue | recA1 endA1 gyrA96 thi-1 hsdR17 supE44 relA1 lac [F’ proAB lacI q Z∆M15 Tn10 (Tetr)] | Stratagene |
JCL16 | BW25113/F’ [traD36 proAB + lacI qZ∆M15 (Tetr)] | [9] |
JCL299 | JCL16 ∆ldhA ∆adhE ∆frdBC ∆pta | |
Plasmid | ||
pRW13 | FRE ackA ::atoB (EC) adhE2 (CA) crt (CA) hbd (CA); ColE1 ori; Ampr | This study |
pRW14 | FRE adhE ::atoB (EC) adhE2 (CA) crt (CA) hbd (CA); ColE1 ori; Ampr | This study |
pRW15 | FRE frd ::atoB (EC) adhE2 (CA) crt (CA) hbd (CA); ColE1 ori; Ampr | This study |
pRW16 | FRE ldhA ::atoB (EC) adhE2 (CA) crt (CA) hbd (CA); ColE1 ori; Ampr | This study |
pRW17 | FRE ackA ::fdh (CB); pSC101 ori; Cmr | This study |
pRW18 | FRE adhE ::fdh (CB); pSC101 ori; Cmr | This study |
pRW19 | FRE frd ::fdh (CB); pSC101 ori; Cmr | This study |
pRW20 | FRE ldhA ::fdh (CB); pSC101 ori; Cmr | This study |
pRW21 | FRE ackA ::ter (TD); Cola ori; Kanr | This study |
pRW22 | FRE adhE ::ter (TD); Cola ori; Kanr | This study |
pRW23 | FRE frd ::ter (TD); Cola ori; Kanr | This study |
pRW24 | FRE ldhA ::ter (TD); Cola ori; Kanr | This study |
pEL11 | PLlacO1:: atoB (EC) adhE2 (CA) crt (CA) hbd (CA); ColE1 ori; Ampr | [15] |
pIM8 | PLlacO1:: ter (TD); Cola ori; Kanr | [15] |
pCS138 | PLlacO1:: fdh (CB); pSC101 ori; Cmr | [15] |