Fig. 5

Antibody screening of the supernatants of controls and enzyme reactions. The various sample preparations are indicated at the top of the figure. Supernatants were applied to the ELISA plates (300 ng glucose equivalents/well) and allowed to dry overnight. ELISAs were carried out on replicates 1 and 2 in the same way as those done in the glycome profiling experiments described in Fig. 3. The glycan binding specificities of different antibody clades are shown at the right [45], with the following abbreviations: XG xyloglucan, HG homogalacturonan, RG rhamnogalacturonan, and AG arabinogalactan. Further information on the binding specificities of the antibodies is provided in the Additional file 1: Table S1. The color scheme of the heatmaps depicts mAb binding strengths with dark blue representing no binding, red intermediate, and bright yellow strongest binding