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Fig. 10 | Biotechnology for Biofuels

Fig. 10

From: Identification and characterization of the glucose dual-affinity transport system in Neurospora crassa: pleiotropic roles in nutrient transport, signaling, and carbon catabolite repression

Fig. 10

Evidence that non-transporting HGT-1/-2 analogs are functional in CCR signal transduction in Δ2 hgt. a Structural models of HGT-1/-2 and GLT-1 based on TMHMM. One arginine that was replaced by lysine is marked with an asterisk. b Growth of EBY.VW4000 harboring the indicated wild-type transporters and corresponding point-mutated analogs on maltose or glucose as the sole carbon source. Each transporter analog was tested using two biological replicates. c Protein concentrations of Avicel cultures of the Δ2hgt mutant expressing one indicated analog controlled by the ccg-1 or cbh-1 promoter. Average values and standard deviations of triplicate are shown. d Protein concentrations, e secretomes, and f growth phenotypes on Avicel plus 2-DG of the mutant cultures in panel C

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Biotechnology for Biofuels and Bioproducts

ISSN: 2731-3654

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