T-6b allocates more assimilation product for oil synthesis and less for polysaccharide synthesis during the seed development of Arabidopsis thaliana

Table 1 Primer details

Primer name	Gene name	Primer sequence (5′-3′)
Primers for semi-quantitative/quantitative PCR
QIKU1F	IKU1	AGTTTTGGTCTAATACAGCTGAG
Q-IKU1R	IKU1	GGTTGAGACTGAGACTGAGATT
Q-IKU2F	IKU2	CGTGTGAGACAAGCGTTAGC
Q-IKU2R	IKU2	GAGGAGACTTGTCCGTGCAT
Q-A/N-InvAF	A/N-InvA	CATACATTACAGCTTCAGAGTTGGG
Q-A/N-InvAR	A/N-InvA	GAGAATAATCCACCACAAACCAGAA
QSBE2.1F	SBE2.1	ATCATGGACTGCAGGTCGAAT
QSBE2.1R	SBE2.1	TCCCGCTAACATCTTCGCCG
QSUS2F	Sus2	AAGCAAGAACAATGTTGGGCA
QSUS2R	Sus2	GCTCAGTAAACCAACATGCTCATC
QAPS1F	APS1	TTCCTGATTTTAGTTTCTATGACCG
QAPS1R	APS1	TGATGAATTTTGCAGTTCTTGATAA
QWRIF	WRI1	AATTTTCCGGCAGAGACGTACA
QWRIR	WRI1	CCTCCTGCGTATTATAGGTGCC
QGL2F	GLABRA2	GAAGCTCGTCGGCATGAGTGGG
QGL2R	GLABRA2	TCTCTCGATTTCACTGTCTGGATTG
QDGAT1F	DGAT1	TTTGGTTAAACATATTGGCAGAGC
QDGAT1R	DGAT1	AATGATAATGGCGAGTGTCTTTGGT
Q-MINI3F	MINI3	TTTGATGATATTGCAACGGAA
Q-MINI3R	MINI3	GATCCTTTGTGTCTTGCTTGT
Actin2F	Actin2	ATGGCTGAGGCTGATGATATTCAAC
Actin2R	Actin2	TCTCAGCACCAATCGTGATGACTTG
QUBQ10F	Ubiquitin10	AGGTACAGCGAGAGAAAGTAGCA
Q-UBQ10R	Ubiquitin10	TAGGCATAGCGGCGAGGCGT
Q6bF	T-6b	GACGAGATCAACGGTGCAAG
Q6bR	T-6b	TGACAAGGTCTCCGAACTGG
Primers used in the construct
Gt1F	Gt1 promoter	GGAATTCCAGGTCATAGGGAGAGGGAGCTTTTG
Gt1R	Gt1 promoter	TTGGCGCGCCAAGTTGTTGTAGGACTAATGAACTGAA
6bF	T-6b	TTGGCGCGCCAAATGACGGTAGCCAATTGGCAGGTTC
6bR	T-6b	CCTTAATTAAGGCTATGCCGAAAGACGGCTTGACCCT

ISSN: 2731-3654