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Fig. 7 | Biotechnology for Biofuels

Fig. 7

From: A versatile 2A peptide-based bicistronic protein expressing platform for the industrial cellulase producing fungus, Trichoderma reesei

Fig. 7

Fluorescence signal intensity of individual transformants and correlation with expression of Cel7A by western blotting. a Transformants for Cel7A-2A-eGFP were analyzed for fluorescence intensity and Cel7A expression. b Transformants for eGFP-2A-Cel7A were analyzed for fluorescence intensity and Cel7A expression. Fluorescence intensity was measured using FLUOstar Omega plate reader (BMG Labtech GmbH) and is represented using arbitrary fluorescence units. Western blotting was performed on cell-free media as shown in Figs. 4 and 5. Each bar represents data obtained for a single transformant colony. Blue bars indicate transformants showing no Cel7A expression. Yellow bars indicate transformants showing Cel7a expression. Red bars represent control strains. Controls: JLT102A (AST1116 expressing native Cel7A under the eno promoter), AST1116 (Cel7A-deleted T. reesei QM6a strain), and SV004 (AST1116 expressing Cel7A-2A-eGFP; colony A5-4 in Fig. 3a)

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Biotechnology for Biofuels and Bioproducts

ISSN: 2731-3654

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