Fig. 5
Production of enzymes by VTT-BR-C0020 in shake flasks on an inducing medium as compared to the parental strain VTT-BR-C0019. T. reesei strains VTT-BR-C0020 and VTT-BR-C0019 were cultivated in shake flasks on an inducing medium (1% yeast extract, 4% milk whey, 4% Avicel). a Culture supernatant samples from the last cultivation day (day 10) visualized on a SDS-PAGE gel. Left lane Parental strain VTT-BR-C0019, right lane VTT-BR-C0020. Additional band corresponding to the heterologous beta-glucosidase is marked with an asterisk. b Extracellular protein measured from cultivation samples of the parental strain VTT-BR-C0019 and VTT-BR-C0020. c β-Glucosidase activity measured from cultivation samples of the parental strain VTT-BR-C0019 and VTT-BR-C0020