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Fig. 2 | Biotechnology for Biofuels

Fig. 2

From: Expression of a heat-stable NADPH-dependent alcohol dehydrogenase from Thermoanaerobacter pseudethanolicus 39E in Clostridium thermocellum 1313 results in increased hydroxymethylfurfural resistance

Fig. 2

Confirmation of BdhA expression in C. thermocellum. Total cell lysates prepared from mid-log and stationary phases were electrophoresed either for SDS-PAGE analysis with Coomassie blue staining (a) or for Western blot analysis (b) probed with His-tag antibody. C JWCT02 (ΔpyrF + pDCW89); 1 JWCT06 (ΔpyrF + pSKW01); 2 JWCT07 (ΔpyrF + pSKW02); 3 JWCT08 (ΔpyrF + pSKW04); M1 Pre-stained SDS-PAGE standards, Broad range (Bio-Rad Laboratories); M2 MagicMark™ XP Western Protein Standard (Invitrogen)

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Biotechnology for Biofuels and Bioproducts

ISSN: 2731-3654

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