Fig. 2

Characteristic growth and enzyme production properties of T. reesei, A. terreus, and P. verruculosum. a Biological triplicates of oxygen transfer rate (OTR) and c cumulative oxygen transfer. For clarity of the depicted values, only every third measuring point of OTR and every eighth measuring point of cumulative oxygen transfer over time are represented by a symbol. b pH value; d Protein content in the culture supernatant; e Residual cellulose concentration; f Standard filter paper activity; RAMOS flasks were cultivated for OTR assessment without interruption. For offline analysis in b, d–f, shake flasks run in parallel, inoculated from the same master mix, were harvested. Error bars represent standard deviation of technical triplicates from pooled biological duplicates. Culture conditions: modified Pakula medium with 5 g L⁻¹ glucose and 30 g L⁻¹ α-cellulose, 250-mL flask, filling volume 20 mL, shaking frequency 350 rpm, shaking diameter 50 mm, inoculum 10⁶ spores mL⁻¹, and 30 °C