Fig. 3

Activity of MtLPMO9B towards amorphous cellulose in the presence and absence of MtPPO7. HPAEC elution pattern of regenerated amorphous cellulose (RAC; 1.5 mg mL⁻¹) incubated with MtLPMO9B (5.0 μg mL⁻¹) with (yellow) and without (red) MtPPO7 (5.0 μg mL⁻¹) addition in the presence of a guaiacol (no. 9 specified in Table 1, 2 mM) and b 3-methylcatechol (no. 17 specified in Table 1, 2 mM) after 24 h. The incubation of RAC with MtLPMO9B results in the formation of non-oxidized gluco-oligosaccharides (GlcOS_n) and C1-oxidized gluco-oligosaccharides (GlcOS ^#_n ). Based on HPAEC, integrated peak areas are shown as the total sum of released non-oxidized (shaded red and yellow) and C1-oxidized (red and yellow) gluco-oligosaccharides after incubation of RAC (1.5 mg mL⁻¹) with MtLPMO9B only (red bars, 5.0 μg mL⁻¹) and MtLPMO9B together with MtPPO7 (yellow bars, 5.0 μg mL⁻¹) in the presence of c guaiacol and d 3-methylcatechol. All incubations were performed in duplicate, and the standard deviations are presented as error bars. See “Methods” for data analysis and details