Boosting LPMO-driven lignocellulose degradation by polyphenol oxidase-activated lignin building blocks

Table 1 RAC incubated with MtLPMO9B only and with AbPPO or MtPPO7 addition in the presence of 21 reducing agents

Gr.	Sub-group	No.	Reducing agent	Activity [%] MtLPMO9B^a	Activity [%] MtLPMO9B + AbPPO^b	Activity [%] MtLPMO9B + MtPPO7^b
I	a	1	4-Hydroxybenzoic acid	100	119	90
		2	Ortho-cresol	100	778	2
		3	Para-coumaric acid	100	9938	2851
		4	Phenol	0^c	>0 ^c	0^c
I	b	5	3-Hydroxy-4-methoxycinnamic acid	100	151	7558
		6	4-Hydroxy-3-methoxyphenylacetone	100	117	131
		7	Coniferyl aldehyde	100	128	562
		8	Ferulic acid	100	107	231
		9	Guaiacol	100	461	5143
		10	Hesperidin	100	56	771
		11	Homovanillic acid	100	156	580
		12	Vanillic acid	100	196	495
I	c	13	4-Allyl-2,4-methoxyphenol	100	89	108
		14	Sinapic acid	100	110	195
		15	Syringic acid	100	107	142
II	a	16	3,4-Dihydroxybenzoic acid	100	99	139
		17	3-Methylcatechol	100	58	104
		18	caffeic acid	100	55	122
II	b	19	3,4-Dihydroxy-5-methoxybenzoic acid	100	99	22
II	b	20	3,4-Dihydroxy-5-methoxycinnamic acid	100	99	116
III		21	Gallic acid	100	105	124

Division based on functional groups of reducing agents (Fig. 2)
^aTotal release of non-oxidized and C1-oxidized gluco-oligosaccharides from RAC incubated with MtLPMO9B set to 100%
^bIncreased (italics ≥50% increase) or decreased percentage of released non-oxidized and C1-oxidized gluco-oligosaccharides from RAC incubated with MtLPMO9B with addition of either AbPPO or MtPPO7 compared to the release of non-oxidized and C1-oxidized gluco-oligosaccharides from RAC incubated with MtLPMO9B alone. Sum of areas of released non-oxidized and C1-oxidized gluco-oligosaccharides are shown in Fig. 2. See “Methods” for more information
^cNo reference due to absent activity of MtLPMO9B towards RAC in the presence of phenol (Fig. 2)

ISSN: 2731-3654