Integrated analysis of 454 and Illumina transcriptomic sequencing characterizes carbon flux and energy source for fatty acid synthesis in developing Lindera glauca fruits for woody biodiesel

Table 2 Statistics of trimmed reads and unigenes of L.glauca fruits by different sequencing strategies

Sequencing strategy and assembly	Trimmed reads		Unigenes
Sequencing strategy and assembly	Number	Mean length (bp)	Number	Mean length (bp)
Illumina sequencing
50 DAF	27,921,143	92.85	130,827	608.44
125 DAF	29,036,556	92.63
150 DAF	24,297,910	92.12
454 sequencing	957,341	518.48	70,432	822.74
Short- and long-read assembly			60,031	1061.95

Three representative fruit samples from 50, 125 and 150 DAF and one mixed sample from seven different developing stages were selected for Illumina and 454 sequencing, respectively. The unigenes separately obtained from long read and short read (201,259 in total) was reconciled by TGICL software

ISSN: 2731-3654