Genome-wide characterization of cellulases from the hemi-biotrophic plant pathogen, Bipolaris sorokiniana, reveals the presence of a highly stable GH7 endoglucanase

Table 1 Effect of metal ions, salts, ionic liquids and detergents on the specific activity of BsGH7-3 and measured by standard spectrophotometric assay

Reagents	Specific activity (%)
10 mM metal ions
Magnesium chloride	91 ± 7
Copper sulphate	100 ± 1
Manganese chloride	444 ± 3
Ferrous sulphate	304 ± 2
Manganese chloride + ferrous sulphate	512 ± 5
Ferric chloride	179 ± 1
Calcium chloride	113 ± 5
Nickel sulphate	114 ± 4
Zinc acetate	97 ± 2
4000 mM salt
Potassium chloride	124 ± 3
Sodium chloride	109 ± 2
20% ionic liquid
[C₂C₁im][C₂C₂PO₄]	112 ± 3
[C₂C₁im][MeCO₂]	122 ± 2
[C₂C₁im][Cl]	107 ± 3
7 mg mL⁻¹ detergents
Ariel™	62 ± 5
Tide™	69 ± 4
Sunlight™	74 ± 4
SDS	51 ± 3

ISSN: 2731-3654